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RT—PCR

网络逆转录聚合酶链反应；逆转录聚合酶链式反应；反转录聚合酶链反应

RT—PCR

网络释义

RT—PCR

逆转录聚合酶链反应

以逆转录聚合酶链反应 (RT PCR)从HIV 1感染者外周血淋巴细胞中扩增抗体轻重链可变区基因 ,插入载体 pCOMB3 ,建立噬菌体 …

逆转录聚合酶链式反应

分别于CPB前后取右心耳组织约5 0mg ,逆转录聚合酶链式反应 (Rt PCR)测其中AT1、AT2受体的基因表达。3-(3-Chloro-1,2,5-th…

反转录聚合酶链反应

应用反转录聚合酶链反应 (RT PCR)法从MES 2 3 5细胞系中克隆了编码TH的cDNA ;结构分析表明 ,其cDNA编码区由 14 97碱基 …

逆转录多聚酶链反应

方法　采用逆转录多聚酶链反应 (RT PCR)检测方法 ,考察了hCG对JEG 3绒癌细胞系基质金属蛋白酶转录表达的影响。结果　绒癌 …

半定量逆转录聚合酶链反应

方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计 …

反转录聚合酶链式反应

目的, 采用反转录聚合酶链式反应(RT PCR), 早期、快速、准确、灵敏地检出MV, 通过对扩增产物的基因分析, 判断传染源和传 …

例句：

After a predetermined time , the total RNA was isolated from cells and the differential mRNA expressions were evaluated by RT-PCR method .

在预定的时间，总 RNA 分离细胞和基因表达的差异进行了评价用 RT-PCR 方法。

In Mexico itself, a lack of RT-PCR equipment could be behind the confusion over the number of cases and deaths caused by the virus .

在墨西哥，缺乏 RT-PCR 设备可能是混淆了由该病毒造成的病例和死亡数量的原因之一。

The technology of RT-PCR proves to be a rapid, accurate and reliable way to do genetic subtyping of influenza A viruses .

逆转录聚合酶链式反应技术为禽流感病毒的分型提供了一种快速、可靠、准确的方法。

RT- PCR assays were used to determine expression of CVB_3 - associated genes in cardiomyocytes .

运用逆转录聚合酶链式反应测定心肌细胞内病毒相关基因的表达。

However , it was recently reported that EU genotype PRRSV was positive by RT-PCR from submitted clinical samples suggestive of PRRS.

然而，近来国内有报道称从临床病料中通过 RT-PCR 方法检测到了欧洲型 PRRSV 的存在。

Canine brain samples from endemic areas were also collected for antigen detection using immunofluorescence assay (IFA) and RT-PCR .

采集疫区犬脑组织，用免疫荧光法和 RT-PCR 法检测狂犬病病毒抗原。

Methods After amplifying the virus by cell culture , influenza virus was differentiated by using RT-PCR and hemagglutination inhibition test .

方法用常规细胞培养法增殖病毒后，分别用 RT-PCR 反应法和常规血凝抑制法进行流感病毒鉴定。

Establishing the technique by using reverse transcription polymerase chain reaction(RT-PCR) to detect one virus or several viruses .

同时建立了马铃薯病毒的单重、双重、多重反转录聚合酶链式反应检测体系。

The results of RT-PCR and RT-PCR-Southern blot show that the target gene had been transcripted.

杂交表明扩增出来的条带为转入的目的序列而非水稻中的同源序列。

The viral RNA was amplified by a reverse transcription polymerase chain reaction (RT-PCR ).

用逆转录 -聚合酶链反应（RT-PCR ）法进行检测。

It was put forward that RT-PCR is a fast and accurate method to diagnose atypical hog cholera .

指出反转录 -聚合酶链式反应（RT—PCR ）是快速确诊非典型猪瘟的诊断技术；

G1 gene sequence of M fragment from hantavirus genome was amplified by reverse transcription polymerase chain reaction (RT-PCR) and analyzed.

采用逆转录聚合酶链反应（RT-PCR ）扩增汉坦病毒基因组 M 片段 G1 区基因序列并测序。

RT-PCR and Western -blotting were used to detect the expression quantity of RAMP1.

逆转录 -聚合酶链反应和蛋白印迹法检测 RAMP1 的表达量；

Identified by individual plant testing , analysis of RT-PCR and dot blot , this fragment was only existed in CMS cauliflower knxd612.

单株检测，RT-PCR 分析，斑点杂交鉴定，确定此片段为花椰菜细胞质雄性不育系 knxd612 所特有。

The homologous sequence in rhesus macaque testicle was confirmed by RT-PCR.

RT-PCR 验证了恒河猴睾丸中的同源序列；

Methods The MAL gene expression in 45 cancer patients was detected with RT-PCR and semi-quantitative RT-PCR technique .

方法采用 RT-PCR 和半定量 RT-PCR 方法检测 45 例食管癌组织和癌旁食管黏膜组织中 MAL 基因的表达。

Throat swab specimens of the patients were detected for H1N1 influenza virus by RT-PCR method .

采集病例的咽拭子样品用 RT-PCR 方法进行甲型H1N1 流感病毒核酸检测。

Methods The S1P receptors expression in K562 cells and CML patients'peripheral blood mononuclear cells was determined by RT-PCR assay .

方法利用 RT-PCR 技术检测慢粒细胞系 K562 和慢粒病人外周血单个核细胞中 S1P 受体的表达情况。

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